摘要
背景:DMEM和RPMI-1640是两种最常用的商品化培养基,二者对肝癌细胞生长的影响尚未见直接的对比研究。目的:比较两种常用培养基对人肝癌BEL-7402与HepG-2细胞系体外生长和增殖效果的影响,从中选择更适合的培养基。方法:分别应用高糖DMEM与RPMI-1640完全培养液培养BEL-7402和HepG-2细胞,于培养的0,24,48,72,96,120h用酸性磷酸酶检测法测定细胞生长和增殖速率,并于倒置显微镜下观察细胞的形态。结果与结论:结果发现BEL-7402和HepG-2细胞在RPMI-1640培养基中的生长增殖速率均明显高于DMEM培养基(P<0.01)。镜下观察证实细胞在RPMI-1640培养基中的黏附和伸展状态更好。因此,建议首选RPMI-1640培养基进行肿瘤细胞体外培养。
BACKGROUND： The most popular commercialized media are DMEM and RPMI-1640. Yet direct comparative study of the two popular media on the influence of liver cancer cell growth has not been reported. OBJECTIVE： Compare the effect of two popular culture media on growth and proliferation of human hepatocarcinoma BEL-7402 and HepG2 cells, in order to find a more suitable medium. METHODS： BEL-7402 and HepG2 cells were cultured in complete growth media prepared with either the high glucose-DMEM or the RPMI-1640 medium. Growth and proliferation rates were detected at timed intervals by the acid phosphatase assay. Cellular morphology was observed under an inverse light microscope. RESULTS AND CONCLUSION： Compared with the DMEM medium, BEL-7402 and HepG2 cells cultured in the RPMI-1640 medium showed a significantly higher growth and proliferation rates （P〈0.01 ）. The cells also revealed better adhesive and stretching status in the RPMI-1640 medium, as confirmed under a microscope. The RPMI-1640 medium is the first choice for tumor cell cultures.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第11期2002-2005,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
北京大学985细胞生物学重点学科建设项目~~
