摘要
为了更好地利用农业废弃物,提高其综合利用率,减少传统化学方法及秸秆焚烧过程造成的环境污染,实验对鸡腿菇、黑曲霉和里氏木霉3株产木质纤维素降解酶系的菌株进行混合平板产酶筛选,结果显示鸡腿菇和里氏木霉平板培养相容性良好,且产酶量高。在相容性实验的基础上,对鸡腿菇和里氏木霉的最优产酶条件进行了研究。在最优条件下:鸡腿菇和里氏木霉接种比例按5:2,接种时间间隔为12h,26oC、150r/min下,发酵3d产漆酶活力达3267.2U/mL,比单独发酵提高106%。
In order to enhance the utilization efficiency,reduce the environmental pollution of traditional chemical treatment and the agriculture waste incineration;we studied the ligninase production by Coprinus comatus,Aspergillus niger and Trichoderma reesei through the plate screening.The results showed that C.comatus mixed culture with T.reesei have a good compatibility and higher yields of Laccase.On the basis of this pre-experiment,we studied the optimal conditions of mixed culture for enzyme production. Under the optimal conditions： the inoculation proportion of C. comatus and T. reesei （5：2）, the interval of time （12 h）, the temperature 26℃, the shake rotation speed 150 r/min, fermented for 3 days, the Laccase activity reached 3267.1 U/mL, increased by 106% contrasted with single culture of C. commatus.
出处
《生物工程学报》
CAS
CSCD
北大核心
2009年第12期2008-2013,共6页
Chinese Journal of Biotechnology
基金
安徽省自然科学基金项目(No.070413266X)
安徽省教育厅优秀人才基金(No.2009SQR2162)
合肥学院博士基金项目(No.RC0016)
合肥学院自然科学基金项目(No.09KY11ZR)资助~~
关键词
鸡腿菇
里氏木霉
木质纤维素酶
漆酶
共发酵
Coprinus comatus
Trichoderma reesei
lingo-cellulase
laccase
co-fermentation
