摘要
采用双向电泳技术,对浙江乐清无花纹和有花纹文蛤外套膜的全蛋白进行了研究,并利用质谱和软件对差异蛋白进行了分析。经PDQuest软件分析,在pH4—7,分子量14.3—200kD之间,无花纹文蛤样品中检测到682±24个蛋白质点,有花纹样品检测到600±35个蛋白质点,平均匹配率为84.5%。研究结果显示,两种文蛤共有256个点存在差异表达,这些差异点可能与贝壳的颜色和花纹形成有关。其中98个点为无花纹文蛤特有,57个点为有花纹文蛤特有,另有101个点在表达量上存在两倍以上差异。在无花纹贝壳中发现分子量为42kD左右,等电点为6.4左右,与库蚊的肌动蛋白(分子量为41.7kD,等电点为5.3)匹配率较高;分子量为29kD,等电点为5.6,与飞鱿的肌动蛋白(分子量为29kD,等电点为5.25)匹配率较高的两个蛋白质。在有花纹文蛤中发现了分子量为27kD左右,等电点为4.8,与丽文蛤属的肌浆钙结合蛋白(分子量为20.8kD,等电点为4.98)匹配率较高。
Using two-dimensional gel electrophoresis, the protein of whole mantle of patterned and non-patterned Meretrix meretrix, from Yueqing, Zhejiang, East China, was studied in mass spectrometry. With software PDQuest, in pH 4— 7 and molecular weight between 14.3—200kD, 682±24 protein spots were detected in the non-pattern clams, and for patterns ones, being 600±35. The average matching rate was 84.5%. Different expressions were shown in 256 spots, being probably related to the shell pattern formation, among them 98 were from non-patterned clams, and 57 from the patterned. In addition, expression of over twice as much difference was revealed in 101 spots. Two proteins of high rate were found. One was about 42kD in the patterned clams with isoelectric point at about 6.4, matching Culex actin （molecular weight 41.7kD, isoelectric point of 5.3）; another was 29kD with isoelectric point 5.6 matching flying squid actin protein （molecular weight of 29kD, isoelectric point of 5.25）. For the non-patterned clams, a 27kD molecular weighted one was found with isoelectric point of 4.8, matching well sarcoplasmic calcium-binding protein （molecular weight 20.8kD, isoelectric point of 4.98） in genus Meretrix lusoria.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2009年第6期777-780,共4页
Oceanologia Et Limnologia Sinica
基金
国家高技术研究发展计划(863计划)
2002AA626020号
长江学者和创新团队发展计划资助项目
2007-2009
宁波市科技局项目
2004C0163号
关键词
文蛤
贝壳花纹
外套膜蛋白
双向电泳
Meretrix meretrix, Shell pattern, Mantle protein, Two-dimensional gel electrophoresis
