摘要
研究了转谷氨酰胺酶在聚丙烯微孔膜上的化学固定化的影响因素,确定了最佳固定化工艺条件,即为:第一步光照反应6 min,单体质量分数为20%,第二步光照时间25 min,接枝率最高可达35.2%;己二胺质量分数为25%,胺烷基化时间150min,胺烷基化温度60℃;戊二醛质量分数3%,戊二醛作用时间45 min;酶液浓度10 mg/mL,固定化时间20 h,固定化温度4℃,固定化酶膜的活力最高可达游离酶的45%。并研究了温度、pH、金属离子对固定化酶膜的酶学性质的影响,其贮存性能和操作稳定性也做了初步研究。
The influencing factors of chemical immobilization of transglutamirmse on polypropylene membrane are studied, and the optimal conditions of immobihzation are determined by experiments： （1）after the first photoreaction for 10 minutes with the monomer mass fraction of 20% ,and the second photoreacton for 25 minutes,the grafting degree can reach 35.2% ;（2）with the hexamethylene diamine mass fraction of 25% ,the alkylate reaction for 150 minutes at 60℃ ,3% of glutaraldehyde solution and the glutaraldethde labilization for 45 minutes, the activity of enzyme can be 10 mg/mL, and after enzyme immobilized for 20 hours at 4℃, the highest activity of the membrane can be 45% of that of free enzyme. The tempemtttre,pH value, the influence of metallic ions on the enzymatic properties of membrane with enzyme immobilized, the storage performance and operational stability are also studied.
出处
《现代化工》
CAS
CSCD
北大核心
2009年第12期49-52,共4页
Modern Chemical Industry
基金
国家"863"课题(2006AA10Z329)
关键词
转谷氨酰胺酶
固定化
酶学性质
transglutaminase
immobilization
enzymatic property
