摘要
目的:研究缺血预处理和大剂量地塞米松(dexamethasone,Dex)预处理对大鼠脑缺血再灌注损伤的保护效应,探寻更为有效的脑损伤的防治方法及检测手段。方法:用改良后的线拴法制备大鼠大脑中动脉闭塞(MCAO)模型,用电刺激方法检测大鼠三叉神经皮层诱发电位(TSEP)。结果:随着缺血再灌注损伤的加重,TSEP波幅下降,而潜伏期延长。缺血预处理组和地塞米松预处理组与缺血再灌注组相比,差异均有统计学意义(P<0.01),缺血预处理组和地塞米松预处理组相比差异无统计学意义(P>0.05)。结论:缺血预处理和大剂量Dex预处理能显著减轻脑缺血再灌注损伤,Dex预处理具有良好的防治脑缺血再灌注损伤的作用。TSEP可作为检测脑缺血再灌注损伤及推测神经功能恢复的客观指标。
Objective To investigate ischemie preconditioning and protection effect of large dosage of dexamethasone preconditioning on the cerebral ischemia reperfusion injuries in rats, and seek more effective methods of the cerebral protection and detective means. Method The experimental MCAO model was set up with middle cerebral artery occlusion by modification of Longua with suture method, and the trigeminal somatosensory evoked potential （TSEP）was detected with electrostimulation. Results Increasing with ischemia reperfusion injury,the TSEP＇s amplitude was decreased and the latent period was lengthened. The results showed that ischemia preconditioning group and Dex preconditioning group both showed significant difference compared with ischemia - reperfusion group （ P 〈 0. 01 ）. Contrarily, there was no significant difference between ischemia preconditioning group and Dex preconditioning group（ P 〉 0. 05）. Conclusion Ischemia preconditioning and large dosage of dexamethasone preconditioning may alleviate cerebral ischemia reperfusion injuries, and dexametbasone preconditioning has the satisfactory protective effect of the cerebral ischemia repeffusion injuries. TSEP can detect cerebral ischemia reperfusion injuries and provide an objective reliable index of neural functional recovery.
出处
《吉林医学》
CAS
2009年第23期2936-2938,共3页
Jilin Medical Journal
关键词
地塞米松
脑缺血再灌注
预处理
诱发电位
Dexamethasone
Cerebral ischemia reperfusion injuries
Preconditioning
Evoke potential
