摘要
目的 探讨不同程度面神经失神经支配后口轮匝肌线粒体结构、琥珀酸脱氢酶 (SDH)的变化与面肌功能恢复的关系。方法 制造颞骨内面神经麻痹模型 ,分为面神经压榨 5s组、15s组、3 0s组 ,再分为观察 15d组、3 0d组。应用神经电图测试 5s组、15s组、3 0s组的平均阈值差值。SDH染色后 ,透射电镜观察各 15d组、3 0d组口轮匝肌线粒体结构和SDH阳性反应产物的变化。结果 面神经压榨 5s组的平均神经兴奋传导抑制时间为 (3 5 .2± 16.8)min ;15s组瞬目反射恢复正常需 (4 7.0± 15 .0 )d ;3 0s组半年内未恢复。 15s15d组线粒体嵴断裂 ,SDH阳性反应颗粒减少 ;3 0d组基本恢复正常。 3 0s15d组线粒体空泡变性 ,SDH颗粒明显减少 ;3 0d组的线粒体结构和SDH颗粒有轻度恢复。结论 面神经受损伤时间越长 ,失神经支配程度越重 ,线粒体病变也越重 ,面肌功能恢复越难 。
Objective To explore the relationship between the functional recovery of facial muscles and the changes of the mitochondria structure and succinate dehydrogenase (SDH) of oris muscles after different degree of denervation. Methods Models of facial paralysis were created by pinching the facial nerve for 5s, 15s and 30s, respectively in 72 rats, and then observe them in 15 and 30 days. Eletroneurogram (ENG) was used to measure the a verage threshold differences among the facial muscles of the rats in the three groups. The oris muscle mitochondria structures and their SDH positive products were observed with transmitting electron microscope. Results It was noted that the average nerve excitement inhibition time was 35.2±16.8 min after pinching the facial nerve for 5s. Recovery of blink reflex took a average of 47±15 days for the rats in 15s group, and it failed to recover within 6 months in the 30s group. Electron microscopic studies revealed broken mitochondria crista and decreased SDH positive reaction granules in rats of 15s after 15 days, but they recovered basically after 30 days. Bubble degeneration and decreased SDH granules could be found in rats of 30s group after 15 days, and they were slightly recovered 30 days later. Conclusion The longer time the facial nerve is injured and the more serious the degree of denervation was sustained, the changes of the mitochondria including the changes of SDH would be more serious and more difficult to recover; the facial nerve injury should be managed as early as possible.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2002年第3期130-132,共3页
Chinese Journal of Physical Medicine and Rehabilitation
基金
国家自然科学基金资助项目 (No.39670 779)
