摘要
目的:应用RT-PCR与免疫组织化学技术检测宫颈癌前哨淋巴结微转移,比较2种方法的差异。方法:选取经病理确诊的53例宫颈癌患者的110枚前哨淋巴结,进行病理切片,免疫组织化学染色与RT-PCR检测CK19的表达。结果:53例患者瘤体病灶PCR及免疫组织化学检测显示均有CK19阳性表达,阳性率100.0%。13例(24.52%)患者前哨淋巴结阳性表达,对13例患者的23枚前哨淋巴结进行PCR与免疫组织化学检测,结果有21枚(91.3%)前哨淋巴结PCR阳性;19枚(82.6%)免疫组织化学阳性。40例患者87枚前哨淋巴结阴性表达。有12例患者28枚前哨淋巴结PCR阳性,淋巴结阳性检出率为32.18%;有7例患者11枚前哨淋巴结阳性,阳性检出率为12.64%。PCR与免疫组织化学阳性表达分别为25例(47.16%)和20例(37.73%)。结论:应用RT-PCR和免疫组织化学技术检测宫颈癌前哨淋巴结微转移的敏感性均高于临床病理;RT-PCR技术检测CK19基因判断宫颈癌前哨淋巴结微转移值得临床推广应用。
OBJECTIVE：To investigate RT-PCR and the immunohistochemistry method for detection of sentinel lymph nodes of patients with cervical cancer. METHODS：A total of 110 sentinel lymph nodes from 53 patients were studied. RT-PCR and immunohistochemistry analysis were performed by using cytokeratin 19 （CK19） as the gene and protein marker. RESULTS：CK19 was detected in 53 tumour bodies from 53 patients by PCR and immunohistochemistry. The positive rate of tumour bodies reached to 100.0%. Thirteen patients were pathology positive and the positive rate was 24.52%. Twenty-one in all 23 lymph nodes from 13 patients were PCR positive and the positive rate was 91.3%. A total of 19 lymph nodes were immunohistochemistry positive,and the detection rate was 82.6%. Twenty-eight lymph nodes of all 87 SLNs from 40 patients was PCR positive,and the detection rate was 32.18%. Eleven lymph nodes of all 87 SLN from 40 cases through immunohistochemistry technology were positive,and the positive rate was 12.64%. Twenty-five patients was detected by PCR,and the detection rate was 47.16%. Twenty patients were detected by immunohistochemistry technology,the detection rate was 37.73%. CONCLUSIONS：The sensitivity of RT-PCR and immunohistochemistry technology are signicantly higher than that of the clinical pathology. CK19 gene with the method of RT-PCR is value for the detection of micrometastasis in sentinel lymph nodes with cervical cancer.
出处
《中华肿瘤防治杂志》
CAS
2009年第18期1405-1407,共3页
Chinese Journal of Cancer Prevention and Treatment
关键词
宫颈肿瘤/病理学
淋巴转移
免疫组织化学
cervix neoplasms/pathology
lymphatic metastasis
immunohistochemistry
