摘要
目的了解儿童白癜风患者血清中抗黑素细胞抗体情况及探讨黑素瘤抗原-1(MART-1)与儿童白癜风的关系。方法采集患儿血清,检测自身抗体,根据抗体滴度高低分成两组,与正常人黑素细胞进行孵育,利用MART-1特异性抗体检测孵育后黑素细胞表面抗原MART-1的变化。结果白癜风患儿血清抗黑素细胞抗体滴度升高。与高滴度抗体患儿血清孵育后的MART-1表达明显增加,并能与MART-1特异性抗体相结合。结论部分儿童白癜风是免疫相关的,MART-1与儿童白癜风可能相关联。
Objective To detect the serum levels ofmelanocyte antibodies and explore the relation between melanoma antigen recognized by T-cells （MART-1） and vitiligo in children. Methods The serum samples were collected from children with vitiligo to test the autoantibodies, and divided into low- and high-titer group according to the test results. Melanocytes were incubated with the serum samples, and the changes of melanocyte surface antigen were evaluated using specific MART-1 antibody. Results The serum melanocyte antibody levels in children with vitiligo were significantly higher than those in normal subjects. The expression level of melanocyte surface antigen MART-1 increased obviously after incubation of the melanocyte with high antibody titer serum samples, and MART-1 was found to specifically bind to specific MART-1 antibody. Conclusion Melanocytes MART-1 may correlate to the autoimmune mechanism in children with vitiligo.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第10期2107-2108,2111,共3页
Journal of Southern Medical University
基金
广东省医学科研项目(30307-4320622)
广州市卫生局资助项目(416-4320622)
