摘要
目的探讨不同分子量与浓度的透明质酸钠(SH)对膀胱黏膜损伤后上皮修复的影响。方法将大鼠随机分为A、B、C、D、E、F共6组,每组8只,A组为空白对照组,B组为阴性对照组,C组为小分子量低浓度组,D组为小分子量高浓度组,E组为大分子量低浓度组,F组为大分子量高浓度组。用盐酸损伤大鼠膀胱黏膜后,分别用大、小(1650ku、1100ku)分子量和高、低(0.2%、0.08%)浓度的SH溶液膀胱内灌注,用常规病理染色技术观察膀胱黏膜损伤范围及炎症浸润的改变情况,用免疫组织化学法检测SH对热休克蛋白60、70(HSP60、HSP70)在黏膜中表达的影响。结果镜下病理改变,C、D、E组均有黏膜充血,少量出血,F组为散在小点状出血;HSP60的表达,F组分别与B、C两组相比有统计学意义(P<0.01),D、C两组对比差异有统计学意义(P<0.05),F、E两组相比差异无统计学意义(P>0.05);HSP70的表达,F组分别与B、C、D、E组相比差异均有统计学意义(P<0.01),D、C两组对比差异有统计学意义(P<0.05)。结论增加SH的分子量及浓度对于提高膀胱黏膜损伤后修复的效果是明显的,这可能与增加了SH的黏稠度有关。
Objective To investigate the effects of the various molecular weight and concentration of sodium hyaluronate （SH） on the bladder epithelium after mucosal injury healing. Methods Rats were randomly divided into A, B, C, D,E, F,totally, of 6 groups. A group was used for the blank control group,B for the negative control group, C for the tow concentration of small molecular weight group, D for small molecular weight high-concentration group, E for the large molecular weight of low-concentration group, F for the high concentration of large molecular weight groups of eight eace. Injury rats who were created by hydrochloric acid applied on bladder mucosa. And then,blad- ders were perfused with large and small （ 1 650 ku, 1 100 ku ） molecular weight, high and low （ 0. 2%, 0. 08% ） concentrations of SH solution. We observed the pathological bladder mucosal injury and inflammatory infiltration with conventional staining techniques, and SH of heat shock protein 60,70 （HSP60, HSP70） expressed in the muco- sa was observed by the technique of immunohistochemistry. Results Endoscopic pathological changes, C, D, E group had mucosal hyperemia, a small amount of bleeding, F group, scattered in small punctate hemorrhage. Concerning the results of HSP60 expression, F group compared with B, C groups, there was a significant difference with them（ P 〈 0. 01 ） , and compared with D, C groups, it was a significant difference （ P 〈 0. 05 ）, and compared with F, E groups, it showed no significant difference （ P 〉 0. 05 ）. Concerning HSPT0 expression, compared with the B, C, D, E group,F group showed a significant difference（P 〈0.01 ） ,and compared with D,C groups,it showed a significant difference （ P 〈 0. 05 ）. Conclusion Increasing molecular weight and concentration of SH can evidently improve the repair capability of bladder mucosa after injury, which may be related to an augment of the viscosity of the SH.
出处
《安徽医科大学学报》
CAS
北大核心
2009年第6期691-694,共4页
Acta Universitatis Medicinalis Anhui
