摘要
目的观察美洲大蠊提取物对大鼠膀胱黏膜损伤后修复的影响,并与透明质酸钠进行比较。方法将大鼠随机分为对照组、模型组、美洲大蠊提取物治疗组和透明质酸钠治疗组,每组70只;再按灌注后2、6、12h、1、2、3和5天7个时间点分设7个亚组,每亚组均为10只。用盐酸损伤大鼠膀胱黏膜,分别用0.25ml美洲大蠊提取物和0.25ml透明质酸钠膀胱内灌注修复受损膀胱黏膜。在各个时间点留取大鼠尿液,然后处死大鼠取得膀胱,用ELISA法检测尿白介素-8浓度,用常规病理染色技术观察膀胱黏膜损伤范围及炎症浸润情况,用免疫组织化学法检测热休克蛋白60、70在黏膜中的表达情况。结果美洲大蠊提取物和透明质酸钠均可明显减少大鼠膀胱上皮缺损和炎性细胞浸润(P<0.01),亦可显著降低大鼠膀胱组织热休克蛋白60、70表达(P<0.01),同时降低尿中白介素-8浓度(P<0.01);两种药物作用比较差异未见统计学意义(P>0.05)。结论美洲大蠊提取物可修复损伤的膀胱黏膜,加强膀胱壁的屏障作用,阻止炎症反应,其干预作用与透明质酸钠相似。
Objective To investigate the effect of Periplaneta Americana extraction and sodium hyaluronate on post - injury healing of bladder mucosa in the rats. Methods The rats were divided randomly into control group, model group, Periplaneta Americana extraction group and sodium hyaluronate treatment group, with 70 rats in each group respectively. According to seven time - points including 2h, 6h, 12h, 1 day, 2days, 3days and 5days after instillation, each group was again divided to 7 subgroups which had 10 rats respectively. The injury of bladder mucosa was made using hydrochloric acid. Bladders were instilled with 0.25ml periplaneta Americana extraction and 0.25ml sodium hyaluronate for the healing of injured mucosa respectively. At designated time - points, urine was obtained, and then, bladder was taken after rats＇ scarification. Interleukin -8 concentration in urine was measured using ELISA, and pathological bladder mucosal injury and inflammatory infiltration were observed using conventional staining techniques, and the expressions of heat shock protein 60 and 70 in the mucosa were analyzed by immunohistochemistry. Results Both periplaneta Americana extraction and sodium hyaluronate obviously diminished the defect of rat bladder epithelium and lessened inflammatory cellular infiltration into rat bladder epithelium （P 〈 0.01 ） , markedly decreased expressions of heat shock protein 60 and 70 in rat bladder tissue （ P 〈 0.01 ） , and lowered the interleukin - 8 concentration in rat urine （ P 〈 0.01 ）. There was no statistical difference （ P 〉 0.05 ） in their effects on post - injury healing of bladder mucosa in the rat. Conclusion Periplaneta Americana extraction may repair injured bladder mucosa, strengthen the wall of bladder, and inhibit inflammatory response. And its effects are similar to those of sodium hyaluronate.
出处
《医学研究杂志》
2013年第1期62-65,共4页
Journal of Medical Research
基金
2011年浙江省中医药科技计划基金资助项目(2011ZA001)
关键词
膀胱黏膜损伤
美洲大蠊提取物
透明质酸钠
修复作用
Bladder mucosal injury
Periplaneta Americana extraction
Sodium hyaluronate
Restoration
