摘要
[目的]比较不同时期玉米须多糖含量的差异及蛋白质的纯化。[方法]采用热回流法提取多糖及木瓜蛋白酶除去蛋白质。通过单因素和正交试验找到木瓜蛋白酶的最优条件。[结果]吉林和松原乳熟期多糖含量为9.87%、10.15%,成熟期为4.85%、4.55%,乳熟期平均产率花柱为7.90%,柱头为12.58%,成熟期平均产率花柱为4.91%,柱头为3.51%。酶水解正交试验最优条件:酶底比1.00%,pH值5.5,体系在水浴中水解1.5h,温度为40℃。[结论]乳熟期多糖含量高于成熟期;乳熟期柱头含量最高,酶法可作为玉米须多糖的脱蛋白的工艺,除蛋白率60.31%,损失率为14.71%。
[ Objective ] In order to compare polysaccharides content from different seasons and positions of corn silk and purification of deproteinization. [ Method] Polysaccharide and papain elimination protein were extracted by thermal reflux method. The most superior condition of papain was found through the single factor and the orthogonal experiment. [ Result ] The polysaecharides contents of Jilin and Songyuan in growing season were 9.87% and 10.15% respectively,ripe season were 4.85% and 4.55% respectively ; during the growing season, average yield of style was 7.90%. stigma was 12.58% , during the ripe season, average yield of style was 4.91% , and stigma was 3.51%. The most superior condition of enzyme hydrolysis was the ratio of enzyme and substrate 1.00% , pH value was 5.5, system in water bath hydrolysis was 1.5 h, the temperature was 40 ℃. [ Conclusion ] The polysaccharides content of growing season was significantly higher than ripe season; Stigma content in growing season was highest, enzyme method was more suitable for remove protein , rate of deproteinization was 60.31% and the lose of polysaceharide was 14.71%.
出处
《安徽农业科学》
CAS
北大核心
2009年第34期17082-17084,共3页
Journal of Anhui Agricultural Sciences
基金
吉林大学博士后基金(801070660432)
吉林省教育厅项目(2008182)
吉林化工学院项目(200950)
