摘要
为进一步探讨木属草本自然群、木本自然群中植物的有效成分的差异，本文首次建立了木属植物中６种化学成分的反相高效液相色谱法，对本属１１种植物中该６种成分进行了定量分析。色谱柱为ＳｕｐｅｌｃｏＳＩＬＬＣ１８，流动相为甲醇—１％乙酸—四氢呋喃（５００∶１１０∶１）；检测波长为２０５ｎｍ，流速１２ｍｌ·ｍｉｎ－１，６种成分的线性范围分别为０１３５～２１６０μｇ，０１６３～２６１０μｇ，０１２３～１９６２μｇ，００６８～１０８０μｇ，０１３５～２１６０μｇ，０１３５～２１６０μｇ，回收率分别为８９６４％，８６８８％，９１３４％，９３３１％，８７７２％和９２０８％。结果提示：二萜类成分是草本自然群区别于木本自然群的特征成分，为进一步将木属中草本和木本两个自然群划分为两个独立的亚属提供了化学分类学的依据。
In this paper, an accurate RP HPLC method for the determination of oleanolic acid 28 O β D glucopyranoside (I), oleanolic acid 3 O α L arabinopyranoside (II), 3β hydroxy oleana 11,13(18) dien 28 oic acid (III), ( ent ) pimara 8(14),15 dien 19 oic acid (IV), oleanolic acid (V) and 3 ox oleana 12 en 28 oic acid (VI) in 11 species of Aralia Linn. was described. The analytical column used was Supelco SIL LC 18. The mobile phase was MeOH 1%HOAc THF (500∶110∶1, pH 3 4). The flow rate was 1 2 ml·min -1 . The detection wavelength was 205 nm. The linear ranges of I～ VI were 0 135～2 160, 0 163～2 610, 0 123～1 962, 0 068～1 080, 0 135～2 160 and 0 248～2 646 μg, respectively. The recoveries of I～VI were from 86 88% to 93 31%. Detailed comparison of the diterpenoic acid, triterpenoids and tritersaponins for the group of herbal with those of woody the content of diterpenoic acid in herbal is higher than that of in woody. It showed that the diterponic acid is the specific constituent in the group of herbal, which is the difference to woody. This conclusion provides the evidence of the chemical classification for dividing the groups of herbal and woody into two distinct subgenus.
出处
《药学学报》
CAS
CSCD
北大核心
1998年第12期933-933,共1页
Acta Pharmaceutica Sinica
基金
自然科学基金
