摘要
目的观察新型免疫抑制毒素白细胞介素-2-绿脓杆菌外毒素(interleukin-2-pseudomonas exotoxin 66,IL-2-PE66)对小鼠角膜移植排斥反应的免疫抑制疗效。方法建立小鼠同种异体穿透性角膜移植模型。36只鼠随机分为IL-2-PE66治疗组及生理盐水对照组,临床观察两组角膜植片免疫排斥反应发生的时间和程度,并在术前及术后第10、15、25、35天分别取出鼠眼行组织病理学检查,同时收集外周血行T淋巴细胞亚群及T淋巴细胞集落形成数分析。结果治疗组与对照组植片存活时间分别为(31.2±2.9)d和(15.8±2.1)d,术后第15天治疗组与对照组T淋巴细胞亚群CD4+/CD8+比率分别为(1.26±0.23)和(2.01±0.23),T淋巴细胞集落形成数分别为(201±18.2)和(286±16.8)。结论IL-2-PE66可推迟角膜移植排斥反应的发生时间;有明显减少外周血中辅助T淋巴细胞百分率的作用;并能减弱小鼠外周血T淋巴细胞集落形成能力。提示IL-2-PE66是一种高特异性的免疫抑制剂。
Objective To study the immunosuppressive effect of the interleukin-2-pseudomonas exotoxin 66（IL-2-PE66） on murine corneal allograft rejection.Methods Thirty-six recipient female BALB/c mice received corneal allografts from C57BL/6 mice and were divided randomly into treatment and control groups.The condition of the grafts was observed twice a week.On days 10,15,25 and 35 after the transplantation,the operated eyes were removed for pathological examinations.Peripheral blood samples were also collected for analysis of T cell subsets and T lymphocyte colony forming unit（T-CFU） assay.Results The survival time of corneal allograft averaged 15.8±2.1 days in the control group and 31.2±2.9 days in the treatment group.The CD4＋/CD8＋ of the T cell subsets 15 days after the operation was 1.26±0.23 in the treatment group and 2.01±0.23 in the control group,with T-CFU of 201±18.2 and 286±16.8,respectively.Conclusion IL-2-PE66 can delay the development of corneal graft rejection,significantly reduce the percentage of T helper cells,and weaken the aggregation of the peripheral T cells.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第11期2152-2155,共4页
Journal of Southern Medical University
基金
国家863攻关课题(863-102-11-12)
国家自然科学基金(39800164)
关键词
角膜移植
免疫毒素
移植物排斥
小鼠
corneal transplantation
immunotoxin
allograft rejection
mice
