摘要
麦胚凝集素是麦胚中一种具有生物学活性蛋白质。根据甲壳素和麦胚凝集素的性质,以甲壳素作为吸附固相,建立了从麦胚中提取、分离和纯化麦胚凝集素的绢丝布袋法工艺。试验结果显示,采用40%~45%的饱和硫酸铵制备粗麦胚凝集素样品时,粗品麦胚凝集素溶液与布袋中甲壳素的使用比例在3∶1~1∶1,甲壳素吸附麦胚凝集素的时间为19 h,SDS-PAGE凝胶电泳显示可获得纯化度近100%的麦胚凝集素样品。纯化的麦胚凝集素相对分子质量为20415。
Wheat germ agglutinin is a kind of protein with biological activity.Based on the characteristics of chitosan and wheat germ agglutinin,chitosan was saved as solid phase of absorption,and a spun silk bag method of extracting,separating and purifying agglutinin from wheat germ was developed.The purity of wheat germ agglutinin was identified with SDS gel electrophoresis.Results： Firstly the rough purification of wheat germ agglutinin is conducted with 40%～45% saturated ammonium sulfate,and the ratio of the roughly purified wheat germ agglutinin liquid and chitosan in the spun silk bag is at 3∶1～1∶1,the time of absorbing wheat germ agglutinin is 19 h.The isolated wheat germ agglutinin has a purity of about 100% with a relative molecule weight of 20415.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2009年第11期30-32,共3页
Journal of the Chinese Cereals and Oils Association
基金
江苏省教育厅高新技术产业(JHB04-003)
关键词
麦胚凝集素
甲壳素
分离与纯化
wheat germ agglutinin chitosan isolation and purification
