摘要
为获得高产氨基酰化酶的米曲霉菌株,选用稀土硝酸镧和硫酸二乙酯对米曲霉菌株CICC-2339进行联合诱变,经摇瓶发酵筛选到氨基酰化酶高产菌株SDESLa,其酶活为39.63μmol.g-1.h-1,较出发菌株提高了39.50%,诱变效果显著。
A high-yield aminoacylase strain SDESLa was obtained by compound mutation with DES-rare earth through fermentation screening method.Its enzyme activity was 39.63 μmol·g-1·h-1,which increased 39.50% than the start strain CICC-2339.The effect of mutation was obvious.
出处
《化学与生物工程》
CAS
2009年第11期43-46,共4页
Chemistry & Bioengineering
基金
内蒙古自然科学基金资助项目(200711020206)
关键词
米曲霉
氨基酰化酶
联合诱变
Aspergillus oryzae
aminoacylase
compound mutation
