摘要
对液体培养的米曲霉（Aspergillusoryzae）3042所产生的氨基酰化酶胞内外分布及其酶的最适pH、温度和热稳定性进行了探讨。结果表明：米曲霉3042属于凝聚型菌株，当搅拌速率为90～120r／min时可获得较好的菌球；氨基酰化酶属于胞内酶，存在于细胞内部或分布于细胞壁上；菌体酶活最适pH为7．0，最适温度为55℃。
In submerged cultures,the aminoacylase activities of Aspergillus oryzae 3042 in vivo or in vitro were discussed,and optimal pH and temperature of pellet aminoacylase activity studied. The results showed that, Aspergillus oryzae3042 could from pellet in flask at speed 90~120rpm during submerged cultures. Aminoacylase of Aspergillus oryzae was believed to be a cell bound enzyme, which could exist in the cell or on the cel1 walI. Optimal pH and temperaure of pellet aminoacylase activity were 7.0 and 5.5, respectively
出处
《食品科学》
EI
CAS
CSCD
北大核心
2000年第7期9-13,共5页
Food Science
基金
教育部"跨世纪优秀人才培养计划"基金
关键词
米曲霉3042
液体培养
氨基酰化酶
凝聚型菌株
Aspergillus oryzae3042 Submerged culture Aminoacylase Pelleted form Cell bound enzyme
