摘要
目的:研究淫羊藿5个黄酮苷淫羊藿苷、朝藿定A、朝藿定B、朝藿定C、宝藿苷Ⅰ在大鼠肠道的在体吸收情况。方法:应用大鼠在体肠灌流模型,用高效液相法测定肠灌流液中淫羊藿各黄酮苷的含量,计算各黄酮苷的肠渗透系数。结果:5个黄酮苷在大鼠各肠段的表观渗透系数按十二指肠、空肠、回肠、结肠顺序依次分别为淫羊藿苷:6.365,5.349,1.004,0.601;朝藿定A分别为4.120,3.397,0.401,0.257;朝藿定B分别为:3.645,3.135,0.990,0.434;朝藿定C分别为:3.114,2.882,0.688,0.470;宝藿苷Ⅰ分别为2.324,2.398,1.529,1.045。淫羊藿苷在大鼠肠道会被水解产生2个代谢产物,朝藿定A,B,C各自会被水解产生1个代谢产物,宝藿苷Ⅰ无代谢产物。结论:5个黄酮成分在大鼠肠段不同部位吸收存在差异,各化合物的回肠和结肠肠段表观渗透系数均显著低于十二指肠和空肠肠段,淫羊藿苷,朝藿定A,B,C的肠道代谢产物与其结构与肠黏膜上的酶相关,并且在十二指肠、空肠代谢较多;在回肠、结肠代谢较少。
Objective： To study the absorption and metabolism of five flavonoids of Herba Epimedii （ icariin, epimedin A, epimedin B, epimedin C and baohuoside Ⅰ ） in rat intestine. Method： Using the rat intestinal perfusion model, the contents of the five flavonoids in perfusates were measured by HPLC and the permeability coefficients in different intestines were calculated. Result： The permeabilities of five flavonoids in duodenum, jejunum, ileum, colon according to the sequence were as ： icariin： 6. 365, 5. 349, 1.004, 0.601, epimedinA： 4. 120, 3.397, 0.401, 0.257, epimedin B： 3.645, 3. 135, 0.990 7, 0.434, epimedin C： 3. 114, 2. 882, 0. 688, 0. 470, baohuoside I ： 2. 324, 2. 398, 1.529, 1. 045, respectively. The analysis of the perfusates showed that icariin, epimedin A, epimedin B, epimedin C could be hydrolyzed in intestine. Icariin had two hydrolytic metabolites, whereas epimedin A, B, C only had one hydrolytic metabolite, respectively. Conclusion： The results indicated that the absorption of all the five flavonoids was significantly different in different intestinal segments, and the permeability in ileum and colon was significantly lower than that of the duodenum and jejunum. The metabolites of icariin, epimedin A, epimedin B, epimedin C were related with their structures and enzymes in intestine, and the metabolites were more in duodenum, jejunum, whereas were less in ileum and colon.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第22期2928-2931,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30572372,30973944)
关键词
淫羊藿黄酮苷
大鼠在体肠灌流模型
吸收
代谢
渗透系数
flavonoids in Herba Epimedii
rat intestinal perfusion model
absorption
metabolism： ermeabilitv coefficient
