摘要
本研究旨在从猪胎儿脑组织中分离培养神经干细胞,观察神经干细胞生长特性和体外增殖、分化特点。利用神经干细胞培养体系,从胎龄30 d的猪胎儿脑组织中分离培养神经干细胞并诱导神经干细胞贴壁分化,采用RT-PCR技术检测干细胞和分化细胞表面标志或相关基因。结果成功分离培养出神经干细胞,神经干细胞具有分化潜能。神经干细胞中Nestin表达强阳性,β-actin、DCX、Hes1、Oct4、Desmin、CD-90、Nanog和Sox2表达阳性;体外诱导的神经干细胞可以分化为星形胶质细胞(表达GFAP)、少突胶质细胞(表达GalC)和神经元细胞(表达NF、NSE和MAP2)。结果提示,从猪胎儿脑组织分离神经干细胞具有可行性和有效性,神经干细胞具有自我更新、增殖和分化潜能。
This experiment was conducted to study the charicteristics of porcine neural stem cells.Brains were dissected from 30-day porcine fetuses,enzymatically dissociated,and grown in the presence of epidermal growth factor,basic fibroblast growth factor etc.Neural stem cells were induced to differentiate in vitro.Expanded neural stem cells and differentiated cells were harvested for analysis using reverse transcription-polymerase chain reaction（RT-PCR）.Porcine neural stem cells could be grown as suspended spheres,and they expressed Nestin,β-actin,DCX,Hes1,Oct4,Desmin,CD-90,Nanog and Sox2.They were differentiated into astrocyte（GFAP）,oligodendrocyte（GalC） and neuron（NF,NSE and MAP2）.This study shows that porcine neural stem cells can be cultured over the long term and are able to self-renew and differentiate into neurons,astrocytes,and oligodendrocytes in vitro.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第11期1686-1689,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家科技部"863"计划(2004AA213072)
西北农林科技大学科研专项基金(07ZR001)资助
关键词
神经干细胞
细胞标记
细胞分化
猪胎儿
neural stem cells
cell markers
cell differentiation
porcine fetus
