摘要
从健康的香蕉组织内分离内生细菌,以香蕉枯萎病菌(Fusarium oxysporum f.sp.cubense)4号小种为指示菌,通过初筛、复筛,得到一株分离自根的有较好拮抗作用的细菌,编号为BEB4。结合该菌菌落、菌体特征,生理生化指标及16SrDNA序列分析,初步鉴定该内生菌为枯草芽孢杆菌。经检测,该菌株能产生铁载体。
Endophytic bacteria were isolated from the healthy tissues of banana. A single strain of antagonist bacteria named BEB4 was isolated from the roots with Fusarium oxysporum f.sp.cubense race 4 as bacterial indicator. Observation of morphological, physiological and biochemical characteristics and 16S rDNA sequences analysis showed the BEB4 was Bacillus subtilis. The BEB4 was tested to produce siderophore.
出处
《热带农业工程》
2009年第4期4-8,共5页
Tropical Agricultural Engineering
基金
科技部国家科技支撑计划项目子课题中国热科院人才引进科研启动基金项目资助(No.2007BAD48B05-5)
关键词
香蕉
内生细菌
拮抗作用
铁载体
枯草芽孢杆菌
banana
endophytic bacteria
antagonistic efficacy
siderophore
Bacillus subtilis
