摘要
对辽宁、吉林、山东及黑龙江省的部分蓝莓苗圃及生产园中蓝莓根癌病发生情况、症状特点及危害程度进行了调查,结果表明蓝莓根癌病多见于苗圃及酸度不适的生产园中。在上述地区采集到57份蓝莓冠瘿瘤和10份土壤样品,经过菌株分离、回接寄主后获得19株致病菌。通过菌落形态、菌体特征、生理生化及16SrDNA序列比对,确定蓝莓根癌病是由根癌土壤杆菌(Agrobacterium tumefaciens)侵染所致。
The crown gall disease occurrence was investigated in different blueberry nurseries and fields located in Liaoning, Jilin, Shandong and Heilongjiang provinces. Fifty seven plant samples and ten soil samples were collected at the above areas. Nineteen of them showed pathogenicity through inoculating of healthy blueberry. The pathogen was identified as Agrobacterium tumefaciens by phenotypic features of the cells and colonies, biochemical characteristics and sequence analysis of 16S rDNA.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2011年第3期283-286,292,共5页
Journal of Jilin Agricultural University
基金
农业部
科技部公益性行业科研专项(nyhyzx07-028)
辽宁省百千万人才工程项目(20089211100)
辽宁省博士启动基金项目(20081069)
关键词
蓝莓
根癌病
病原菌鉴定
16S
RDNA序列分析
根癌土壤杆菌
blueberry
crown gall disease
identification of pathogen
16S rDNA sequence analysis
Agrobacterium tumefaciens
