摘要
目的建立同时测定甲硝唑含漱液中甲硝唑和羟苯乙酯含量的方法。方法高效液相色谱法,选用phenomenex-C18（4.6×250mm,5μm）柱,以乙腈∶水（25∶75）为流动相,检测波长为255nm。结果甲硝唑和羟苯乙酯的回归方程分别为：Y=1.3892×10^4X-1.068226×10^4（r=0.9999,n=9）和Y=1.092077×10^5X-2.022870×10^4（r=0.9999,n=9）;线性范围分别为2.7-270.8μg.mL^-1和0.2-53.0μg.mL^-1;平均回收率分别为104.5%和98.3%。结论所用方法简便快速,结果准确可靠,可作为甲硝唑含漱液的质量控制方法。
OBJECTIVE To establish a method for determining simultaneously the contents of metronidazole and ethylparaben in gargle metronidazole.METHODS The HPLC method employed a phenomenex-C18 column with the mobile phase acetonitrile∶water（25∶75）.The detection wavelength set at 255nm.RESULTS The regression equation of metronidazole and ethylparaben were Y=1.3892×10^4X-1.068226×10^4（r2 =0.9999,n=9） and Y=1.092077×10^5X-2.022870×10^4（r2=0.9999,n=9）;the linear range were 2.7～270.8μg·mL^-1 and 0.2～53.0μg·mL^-1;the average recovery （RSD） were 104.5 % （0.4） and 98.3 % （2.3）, respectivery.CONCLUSION This method is simple, accurate, reliable and suitable for the quality control of gargle metronidazole.
出处
《海峡药学》
2009年第10期49-51,共3页
Strait Pharmaceutical Journal
