摘要
利用普通小麦（ＴｒｉｔｉｃｕｍａｅｓｔｉｖｕｍＬ．）高分子量谷蛋白亚基（ＨＭＷＧＳ）多克隆抗体和单克隆抗体，对小麦品质性状及不同麦类作物近缘种属的籽粒贮藏蛋白进行了免疫化学测定，以建立小麦品种性状的快速测定方法并研究不同麦类作物胚乳贮藏蛋白的免疫同源性。结果表明：抗原抗体反应与品质性状的相关性因抗体种类及品质性状的不同而不同，多抗略优于单抗，不同单、多抗间相差较大；籽粒蛋白质含量及干、湿面筋含量与吸附值相关程度较高，与沉淀值则中等，而面包性状相关性较差。多克隆抗体吸附值与籽粒蛋白质含量、湿面筋含量、干面筋含量、面包体积及面包比容的最大相关系数分别为０．７６２０、０．８９４２、０．８８７３、０．６１０３、０．４５９８和０．４７４４，单克隆抗体吸附值与其最大相关系数分别为０．７８３７、０．７７４５、０．７８２２、０．６８４１、０．６８７３和０．５９８２。小麦近缘种属籽粒贮藏蛋白与普通小麦１Ｄｙ１０亚基具有一定程度的免疫同源性，其中以黑麦（ＳｅｃａｌｅｃｅｒｅａｌｅＬ．）、斯卑尔脱（Ｔ．ｓｐｅｌｔａＬ．）、节节麦（ＡｅｇｉｌｏｐｓｓｑｕａｒｒｏｓａＬ．）及圆锥小麦（Ｔ．ｔｕｒｇｉｄｕｍＬ．）与其同源程度较高。
An immunochemical assay using polyclonal and monoclonal antibodies of high_molecular_weight glutenin subunit (HMWGS) in Triticum aestivum L. was carried out to determine the quality of wheat and to investigate the immunological homology of the storage proteins in cereal endosperms in different closely related wheat genera and species. The results showed that correlation between the antigenantibody reaction and the wheat quality varied with the type of antibodies used and the quality. The correlation coeffecient was slightly higher when the polyclonal antibodies were used than monoclonal antibodies were used. The correlation coeffecient was high between the antibody binding and the protein content, and wet/dry gluten content, with Zeleny sedimentation value, while that between antibody binding and bread characters was lower. The highest correlation coeffecient between the polyclonal antibody binding and the protein content in grains, wet and dry gluten contents, bread volume and bread ratio volume was 0.7620, 0.8942, 0.8873, 0.6103, 0.4598 and 0.4744 respectively, while the highest correlation coeffecient between the monoclonal antibody binding and the above parameters was 0.7837, 0.7745, 0.7822, 0.6841, 0.6873 and 0.5982 respectively. The immunological homologies between HMWGS 1Dy10 in common wheat and endosperm storage protein in wheat grains of different genera and species were noticed. The crossreaction among Triticum aestivum L., Secale cereale, T. spelta L., Aegilops squearrosa L. and T. Turgidum L. was stronger than that among other cereals.
基金
国家自然科学基金
关键词
抗体
免疫化学测定
品质性状
免疫同源性
小麦
Antibodies, Immunochemical assay, Highmolecularweight glutenin subunit, Quality, Immunological homology, Wheat
