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Study on Immunochemical Assays for the Organophosphorus Insecticide Chlorpyrifos

Study on Immunochemical Assays for the Organophosphorus Insecticide Chlorpyrifos
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摘要 The anti-chlorpyrifos polyclonal antibodies were obtained by using the artificial immuneantigen to immune in New Zealands white rabbits. The enzyme-tagged antibodies wereprepared by coupling horseradish peroxidase (HRP) to the purified antibody with themodified sodium periodate method. The indirect competitive enzyme linked immuno-sorbentassays (ELISA) and the HRP-tagged antibody direct ELISA (E-Ab) were established, respectively.The limit of detection (LOD) for the indirect ELISA and E-Ab were 0.0033 and 0.0042 gmL-1, respectively. The linear detection ranged well from 0.005 to 2.0 g mL-1. The anti-chlorpyrifos polyclonal antibodies were obtained by using the artificial immuneantigen to immune in New Zealands white rabbits. The enzyme-tagged antibodies wereprepared by coupling horseradish peroxidase (HRP) to the purified antibody with themodified sodium periodate method. The indirect competitive enzyme linked immuno-sorbentassays (ELISA) and the HRP-tagged antibody direct ELISA (E-Ab) were established, respectively.The limit of detection (LOD) for the indirect ELISA and E-Ab were 0.0033 and 0.0042 gmL-1, respectively. The linear detection ranged well from 0.005 to 2.0 g mL-1.
作者 WUGang HUANGYa-li ZHUGuo-nian WUHui-ming LICong
机构地区 ZhejiangEntry-ExitInspection＆QuarantineBureau InstituteofPesticide＆EnvironmentalToxicology
出处 《Agricultural Sciences in China》 CAS CSCD 2004年第5期371-375,共5页 中国农业科学（英文版）
关键词 CHLORPYRIFOS ANTIBODY Immunochemical assays Chlorpyrifos, Antibody, Immunochemical assays
分类号 S482.33 [农业科学—农药学]
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Agricultural Sciences in China
2004年 第5期

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