摘要
从酵母菌中分离出拮抗重金属、经铜诱导产生金属硫蛋白的酿酒酵母（Saccharo－mycescerevisiae）BD101。无细胞抽提液经SephadexG－50、DEAESepharoseCL－4B、SephadexG-25三次凝胶及阴离子交换柱层析分离纯化得到两个金属硫蛋白亚型。Mr约为7kD，由60个氨基酸组成，其中半胱氨酸含量占10％，每分子金属硫蛋白（Cu-MT）含6个分子Cys，可结合4个铜原子。
Selection of Saccharomyces cerevisiae BD101 having resistance to metal ions and producing metallothionein by inducer from Saccharomyces. These proteins were isolated and Purified by Sephadex G-50 and subsequent DEAE Sepharose CL-4B,then Sephadex G-25 for desalination. There were two iso-MTs for Cu-induced. The,molecular weightS were 7 kD and had 60 amino acids. Both Cu-MTs were rich in Cysteine (10%). 4 atom CU / 6 mole Cys / mole CU-MTs.
出处
《微生物学报》
CAS
CSCD
北大核心
1998年第4期289-294,共6页
Acta Microbiologica Sinica
基金
国家自然科学基金!39370021
关键词
酿酒酵母
金属硫蛋白
分离纯化
鉴定
Saccharomyces cerevisiae, Metallothionein, Isolation and Purification
