摘要
本文为真核微生物ＭＴ４５篇文献综述，在酵母菌和真菌中，细胞对重金属毒害的抗性或者以结合金属离子的蛋白质─—ＭＴ形式调控，或者通过合金属离子的整合肽（γ－Ｇｌｎ－Ｃｙｓ）ｕＧｌｙ类型调控。本综述包括酵母菌ＭＴ序列、金属结合特性；ＭＴｓ及ＰＣｓ结构和功能，以及ＭＴ基因、基因放大、基因调控；最后，我们叙述了酵母菌ＭＴ在制药、金属回收和ＭＴ启动子在生物工程中的应用。
A review is reported on metallothionein (MT) from eucaryotic microbial sources with 45 refer ences. In yeast and fungi,cellular resistance to heavy metal cytotoxicity is mediated either by binding metal ions to proteins of the metallothionein type or by chelating to phytochelatin-peptides(PCs) of the general formula (γ-Gln-Cys)nGly. The present review involves yeast metallothionein sequence and metal-binding properties,the MTs and PCs structure and function as well as the structure of the feast CUP1 (MT genes),mechanismg of gene amplification and gene regulation,then,we describe the appli cation of the yesat MTs in medicine、metal recovery and that of CUP1 promoter in biotechnology.
出处
《生物工程进展》
CSCD
1996年第3期33-43,共11页
Progress in Biotechnology
基金
国家自然科学基金
