摘要
利用荧光素标记LZF-ⅠDNA探针对不同培养代龄南方鲶SME-1细胞简单重复DNA进行了检测,获得了清晰的DNA指纹图谱。发现:随着细胞代龄的增加,同一代龄细胞群体内和不同代龄细胞群体间共有谱带概率、相似系数、最大等位基因频率均明显下降,而突变率则随着代龄增加而增加。利用DNA指纹图技术分析SME-1细胞遗传物质的变化是可行的。为SME-1细胞进一步的培养,并为SME-1细胞的研究利用提供了分子遗传学资料。
The simple repetitive DNA of SME-1 cells at different passages was detected byflourescein labelled probe LZF-Ⅰ. From the DNA fingerprints, some results could beconcluded: When the passage rised, the probability of bands sharing, the maximumallelic frequency and the similarity coefficient decreased between cells at the samepassage and at the different passage, while the mutation rate increased as variation ofcells strengthed. Therefore, to analyse the genetic variation of SME-1 cell line byDNA fingerprinting was feasible. These papers seemed to provide a good detectivemethod and genetical markers for clone culture of SME-1 cells, and also provide amolecular basis for study of SME-1 cell line in cell biology.
