摘要
鱼类肝细胞分离与原代培养技术已有30多年的历史,目前已有40多种鱼的肝细胞被分离、培养并用于各种研究中。主要讨论鱼类肝细胞的分离与培养方法以及不同条件对细胞生理的影响;概括鱼类肝细胞体外培养在生理学、环境毒理学和药理学等方面的应用研究,旨在为建立更多的鱼类肝细胞原代培养模型并应用于相关的研究领域提供参考依据。
For more than 30 years primary culture technique of fish heaptocytes has been utilized exten- sively and over 40 fish species studied with respect to the isolation and cultivation of hepatocytes. The review discusses procedures for isolation, primary culture of fish hepatocytes and the influence of different culture conditions on the physiology of the cells ; meanwhile the application of ecto - culture of fish heaptocytes culture in physiology of fish, environmental toxicology and pharmacology was sumarized. This paper aims to provide referential information for the establishment of more primary culture models of fish hepatocytes and application in related research.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2012年第1期147-157,共11页
Acta Agriculturae Universitatis Jiangxiensis
基金
科技部国际科技合作项目(2009DFA32620)
中央级公益性科研院所基本科研业务费专项基金(2011JBFC05)
无锡市科技计划(国际科技合作)(CZE00906)
关键词
鱼类
肝细胞
原代培养
细胞分离
Fish
hepatocytes
primary culture
cell isolation
