摘要
目的探讨输注自体骨髓间充质干细胞延长犬活体肝移植存活的机制。方法14只受体犬建立活体肝移植模型后,随机分为对照组和处理组,对照组仅做活体肝移植,处理组在活体肝移植的基础上,术中输注自体骨髓间充质干细胞,术后观察两组犬的生存时间、肝功能(AST、ALT)、组织病理变化及移植细胞的分化状况。结果①处理组比对照组的中位生存时间明显延长(P<0.01)。②肝功能变化:术后两组AST、ALT总体相比较,处理组AST、ALT水平降低,有显著性差异(P<0.01)。③组织病理变化:术后两组均出现不同程度的免疫排斥反应,且排斥程度相似。④移植细胞分化情况:输注的骨髓间充质干细胞向有功能的肝样细胞分化。结论输注自体骨髓间充质干细胞可以延长活体肝移植受体犬的存活时间;经门静脉输注的自体骨髓间充质干细胞可向肝样细胞分化。
Objective To investigate the mechanism ofantologous mesenchymal stem cells （MSCs） in prolonging the survival of dogs receiving living donor liver transplantation. Methods Canine models of allogenic living donor liver transplantation was established in 14 beagle dogs by non-venous by-pass method, and in 7 of the recipients, autologous MSCs labeled by BrdU was infused into the portal vein, with the other 7 dogs as the control. The survival time of the two groups of the dogs was observed after the operation. The liver function （AST and ALT levels）, liver pathologies and the differentiation of the transplanted cells were also evaluated postoperatively. Results Compared with the control group, the dogs receiving MSC transplantation showed significantly increased median survival time （P〈0.00 1） with lowered levels of AST and ALT （P〈0.01）. The two groups exhibited similar graft rejection after the operation. In dogs with MSC transplantation, the BrdU-labeled MSCs differentiated into liver-like cells in the liver and secreted albumin. Conclusions Autologous MSCs infusion through the portal vein during allogenic living donor liver transplantation can prolong the survival of the recipient dogs. The stem cells transplanted can differentiate into mature liver-like cells and secrete albumin in the hepatic tissue.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第9期1783-1786,共4页
Journal of Southern Medical University
基金
国家高技术研究发展(863)计划项目(2006AA02A141)
关键词
活体肝移植
动物模型
犬
骨髓间充质干细胞
长期存活
living donor liver transplantation
animal model
canine
mesenchymal stem cells
long-term survival
