摘要
目的研究低频电刺激对急性局灶性脑梗死大鼠运动功能和梗死边缘区胶质纤维酸性蛋白（GFAP）表达的影响，探讨低频电刺激治疗促进脑梗死后运动功能恢复的机制。方法54只成年雄性SD大鼠，随机分为低频电刺激组、模型对照组及假手术组，每组18只，每组动物再分为治疗3，7和14d3个时间点，每个时间点6只。参照Longa等的线栓法制成左侧大脑中动脉闭塞（MCAO）模型。低频电刺激组于造模术后3d开始进行低频电刺激治疗，模型对照组应用相同的低频电刺激仪治疗，但不予以电流刺激，假手术组不予任何治疗。分别在治疗前和治疗后各时间点给予平衡木行走测评、转棒上行走测评和网屏试验等功能评定；以免疫组织化学技术观察梗死边缘区胶质纤维酸性蛋白（GFAP）的表达水平变化。结果低频电刺激组大鼠运动功能较模型对照组明显改善（P〈0．05）。低频电刺激组缺血性半影区的GFAP阳性率较模型对照组高（P〈0．05）。结论低频电刺激能促进脑梗死大鼠运动功能恢复，其中一个重要机制可能是低频电刺激能增强GFAP的表达，促进缺血后脑的可塑性变化，构成了脑梗死后功能恢复的物质基础。
Objective To study the effects of low-frequency electrical stimulation（LFES） on motor func- tion and the expression of glia fibrillary acidic protein （GFAP） around cerebral infarction sites in rats. Methods Fifty-four male adult Sprague-Dawley rats were randomly divided into a LFES group, a placebo group and a sham operation group （ 18/group）. All groups were randomly divided into 3 treatment groups. A rat model of middle cer- ebral artery occlusion （MCAO） was established using intraluminal filament occlusion. Treatment was carried out 3 d after the operation. Rats in the LFES treatment groups were stimulated with LFES for 3, 7 or 14 days （ 10 rain/d） ; the placebo groups were treated in the same way without electric stimulation; the sham operation sub- groups didn＇t receive any therapy. Scores on a beam-walking test, a rotating pole test and a screen test were as- sessed at each time point mentioned above. Expression of GFAP was also assessed using immunohistochemcal tech- niques. Results The paralysed limbs recovered motor function better in the LFES groups than in the control groups. GFAP-positive ceils were more numerous at the margins of the infarction area in the treated groups than in the control groups. Conclusions LFES might increase the expression of GFAP, which might be an important mechanism in improving brain plasticity after cerebral isehemia, aiding the recovery of the central nervous system and rebuilding its functioning.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2009年第10期655-658,共4页
Chinese Journal of Physical Medicine and Rehabilitation
基金
基金项目：国家自然基金资助项目（30772304）
广东省自然科学基金项目（8451008901000885）
关键词
低频电刺激
脑梗死
运动功能
胶质纤维酸性蛋白
Low-frequency electric stimulation
Cerebral infarction
Motor function
Glia fibrillary a- cidic protein
