摘要
目的：研究高血脂对大鼠脑缺血再灌注后海马CA4区内星形胶质细胞表达胶质纤维酸性蛋白（GFAP）的影响。方法：高脂饮食建立高血脂模型。以线栓法制作大鼠大脑中动脉阻塞的局灶性脑缺血再灌注模型，采用免疫组织化学和蛋白印迹与神经行为相结合的方法，观测缺血再灌注侧大脑海马CA4区内星形胶质细胞GFAP的表达和神经功能的变化。结果：在相同时间点内与假手术组比较，两组脑缺血再灌注组的星形胶质细胞数量均增多，GFAP的表达均增高；与脑缺血再灌注组比较，高血脂合并脑缺血再灌注组的星形胶质细胞数量减少，胞体较小，突起也少而细，GFAP的表达减少。结论：脑缺血再灌注损伤后星形胶质细胞的数量、形态以及GFAP的表达呈动态变化。
Objective： To observe the effect of hyperlipemia on glial fibrillary acidic protein （GFAP） expression in the hippocampus CA4 in rats after ischemiareperfusion in the brain. Methods： Application of highfat diet produced lipid model. The focal cerebral ischemicreperfusion model was established with thread embolism of the middle cerebral artery. Immunohisto chemistry, Western blot and neurobehavioral evaluation were used to examine the expression of GFAP in the hippocampus CA4 in the side of cerebral ischemia reperfusion and neurological function disorders. Results： Compared with shamoperation group, the number of astrocyte and the expression of GFAP were increased in both cerebral ischemia reperfusion groups in the same time point. Compared with cerebral ischemia reperfusion group, the expression of GFAP was decreased, the population of positive astrocyte declined, cell body was small and process was slender in hyperlipemia with cerebral ischemia- reperfusion group. Conclusion： After cerebral ischemia reperfusion, the number and shape of astrocyte and the expression of GFAP present dynamic changes.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2011年第6期766-769,共4页
Chinese Journal of Anatomy
基金
山西省高校科技研究开发项目（200613049）
关键词
胶质纤维酸性蛋白
星形胶质细胞
高血脂
脑缺血再灌注
glial fibrillary acidic proteim astrocyte
high blood lipids
cerebral ischemia-reperfusion
