摘要
通过对11种大孔吸附树脂对比研究,筛选出了一种对胡芦巴中总皂苷具有最佳吸附解吸性能的树脂,并对该树脂的静态和动态吸附性能进行了研究,确定了树脂纯化总皂苷的工艺参数。结果表明,HPD-400A型树脂对总皂苷有良好吸附分离性能,Freundlich等温吸附模型较Langmuir模型更适宜描述树脂对胡芦巴总皂苷的吸附;吸附分离总皂苷的工艺条件:树脂柱径高比为1∶10,上柱液质量浓度为1.358 mg/mL,流速为2 BV/h,解吸流速1 BV/h,解吸液为体积分数60%的乙醇,洗脱剂用量为1.5 BV。经大孔树脂纯化前总固物中总皂苷质量分数为11.38%,纯化后总固物中总皂苷质量分数为42.76%,纯度提高了近4倍。
Eleven types of macroporous resin were compared and a kind of resin with an optimum performance for absorbing and desorbing the total saponins from fenugreek was selected. The static and dynamic adsorption performances of such resin were investigated, and the technical parameters of the purification of saponins were obtained. The results show that HPD400A macroporous resin has a better adsorption and separation performance. Freundlich model is better than Langmuir model for describing the adsorption of saponins. The optimum adsorption conditions are as follows： the diameter/height ratio of resin column is 1 ： 10, the sample mass concentration is 1. 358 mg/mL, the current velocity is 2 BV/h, the desorption current velocity is 1 BV/h, the desorption solution is 60% volume fraction ethanol, the amount of eluant is 1.5 BV. The mass fraction of saponins is 11.38% before purification while the mass fraction of saponins is 42.76% after purification. The purity is increased by almost four times.
出处
《化学工程》
CAS
CSCD
北大核心
2009年第10期5-9,共5页
Chemical Engineering(China)
基金
2006年陕西省资源生物重点实验室访问学者项目:陕西省科技厅重点攻关项目(2007K07-05)
关键词
胡芦巴
总皂苷
大孔吸附树脂
纯化
fenugreek
total saponins
macroporous adsorption resin
purification
