摘要
目的：研究一氧化氮（ＮＯ）抑制单核内皮细胞粘附的机制。方法：以硝普钠作为ＮＯ的供体，用细胞粘附实验、流式细胞仪荧光强度测定、细胞ＥＬＩＳＡ、Ｎｏｒｔｈｅｒｎｂｌｏｔ等方法分别从细胞、蛋白表达、基因表达方面研究ＮＯ对内皮细胞粘附分子（ｖａｓｃｕｌａｒｃｅｌａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅ，ＶＣＡＭ１）表达的影响。结果：ＮＯ抑制ＩＬ１α诱导的单核内皮细胞粘附，抑制ＩＬ１α诱发的ＶＣＡＭ１的表达并呈时间依赖性及浓度依赖性，ＮＯ在粘附分子ＶＣＡＭ１的基因表达水平上亦呈现抑制作用。结论：ＮＯ抑制ＶＣＡＭ１的表达是其抑制单核内皮细胞粘附及抗动脉粥样硬化的机制之一。
Objective: To study the inhibitory mechanism of nitric oxide on adhesion between monocyte and endothelial cells. Methods:Cell adhesion,FACS, cell ELISA and Northern blot were applied to study effects of nitroprusside, a donor of nitric oxide, on expression of protein and mRNA of (vascular cell adhesion molecule, VCAM 1). Results: Expression of VCAM 1 protein and mRNA was inhibited by nitroprusside, in a dose time dependent manner. Conclusion: Inhibitory effect of nitric oxide on VCAM 1 is one of the mechanisms of its antiadhesion between monocyte and vascular endothelial cells,as well as that of its antiatherosclerosis.
出处
《北京医科大学学报》
CSCD
1998年第5期439-442,共4页
Journal of Peking University(Health Sciences)
关键词
一氧化氮
药理学
内皮
细胞粘附
动脉粥样硬化
Nitric oxide/pharmacol Endothelium/drug eff Cell adhesion/drug eff Monocytes/drug eff
