摘要
目的:研究氩氦刀冷冻消融对SD大鼠皮下移植瘤细胞凋亡及T细胞免疫的影响,评价氩氦刀冷冻治疗对大鼠免疫功能的影响.方法:将45只大鼠随机分成3组:正常对照组,荷瘤对照组,冷冻治疗组,每组15只.建立SD大鼠皮下移植瘤动物模型.冷冻治疗组:消融冷冻治疗3-5min,复温时间30s.荷瘤对照组:将氩氦刀置入肿瘤3min,而不予冷冻治疗.正常对照组:正常饲养.分别于冷冻后3,12,24h和3,7d时处死动物,取材.光学显微镜观察冷冻坏死消融规律;末端标记法(TUNEL)原位检测周边冷冻损伤区带细胞凋亡和检测特异性细胞毒性T淋巴细胞的增殖.结果:在冷冻中心区,细胞死亡以坏死为主,在周边冷冻损伤区,光镜形态学检查及TUNEL染色可观察到明确的细胞凋亡,术后12h凋亡达高峰,凋亡率为68.28%±7.85%.氩氦刀治疗后可显著刺激特异性T淋巴细胞增殖,与对照组比较具有显著性差异(P<0.01).结论:氩氦刀通过坏死和凋亡两种途径达到有效的消融,并且可有效激活小鼠肿瘤抗原特异性的T细胞,氩氦冷冻有效地诱发和增强机体的抗肿瘤免疫效应.
AIM： To investigate the effects of argon-helium （Ar-He） cryoablation on cell apoptosis and T-cell immunity in rats subcutaneously implanted with breast cancer cells.METHODS： Forty-five healthy male Sprague- Dawley rats were randomly divided into three groups： normal control group, cancer control group and cryoablation group. A xenograft rat model of breast cancer was established by sub- cutaneous injection of breast cancer cells （W-256）into Sprague-Dawley rats. After argon-helium cryoablation, cell necrosis was observed under light microscopy, cell apoptosis in peripheral cryoablation zone was detected by terminal deoxynucleotidyl transferase-mediated dUTP in situ nick end labeling （TUNEL）, and the proliferation of cytotoxic T lymphocytes was investigated using mixed lymphocyte reaction assay. RESULTS： In central cryoablation zone, necrosis dominated in cell death. In peripheral cryoablation zone, cell apoptosis was definitely observed by morphological examination under light microscopy or TUNEL staining. The apoptosis reached the peak at 12 h after cryoablation, with an apoptosis rate of （68.28 ±7.85）%. Cryoablation could effectively activate the proliferation of cytotoxic T lymphocytes. The proliferation rate of cytotoxic T lymphocytes in the cryoablation group was significantly higher than that in the cancer control group （P 〈 0.01）. CONCLUSION： Argon-helium cryoablation can effectively cause cell death either through inducing necrosis or apoptosis. Cryoablation may induce cell apoptosis in peripheral cryoablation zone. Besides, cryosurgery can effectively activate tumor antigen-specific T lymphocytes and improve T cell-mediated immunity.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第23期2362-2366,共5页
World Chinese Journal of Digestology
