摘要
[目的]观察氩氦冻融的小细胞肺癌细胞NCI-H446对正常人外周血单个核细胞免疫功能的影响。[方法]分离正常人外周血单个核细胞,与氩氦冻融的小细胞肺癌细胞NCI-H446混合培养,设一个空白对照组,培养过程中隔天加入IL-210U,分别在第3、7、14d检测培养上清液中IL-12、IL-10含量及NK细胞、CTL细胞的杀伤活性。[结果]氩氦冻融的肺癌细胞NCI-H446与PBMC混合培养后,第3、7d时,培养上清液中IL-12含量分别为71.8±19.1pg/ml,106.2±13.6pg/ml,对照组IL-12含量为38.5±8.4pg/ml,40.8±8.3pg/ml,试验组较对照组明显升高(P<0.05),在第14d时试验组与对照组IL-12表达相当。IL-10的表达在试验组与对照组之间始终未发现明显差异。试验组NK细胞、CTL细胞的杀伤活性在14d内均明显高于对照组(P<0.05)。[结论]氩氦冻融的肺癌细胞可以上调PBMC对IL-12的表达,而对IL-10的表达无影响,同时可明显增强NK细胞和CTL活性。
[Purpose ] To investigate the immunological response of peripheral mononuclear cell （PBMC） when co-culture with the small cell lung cancer cell NCI-H446 that frozen and thawed by Ar-He targeted cryosurgery equipment. EMethods] After isolated the PBMC cultured with freeze- thaw lung cancer cell. IL-2 that regards as immunoadjuvant was added in the culture system. The level of IL-12 and IL-10 in the supernatant was examined at 3,7,14 days. The activity of NK and CTL was checked at the same time. [Results] The level of IL-12 significantly increased when PBMC were cultured with freeze-thaw cell （P〈0.05）. The level of IL-10 had no difference with the control group. Freeze-thaw cell group increased the activity of CTL and NK cell significantly compared to control group （P〈0.05）. [Conclusion] The freeze-thaw lung cancer cell can enhance the anti-tumor immune of PBMC, which probably depends on increasing of IL-12 and releasing of intracellular antigens
出处
《中国肿瘤》
CAS
2007年第11期895-897,共3页
China Cancer
基金
全军"十一五"医药卫生科研项目(06MB021)
