摘要
目的：观察野生型ｐ５３基因转染的Ｋ５６２细胞对紫外线诱导其细胞凋亡的影响。方法：应用基因转染技术将重组野生型ｐ５３的逆转录病毒载体转导ｐ５３蛋白缺失的Ｋ５６２细胞，用紫外线照射Ｋ５６２ｐ５３细胞不同时间后再培养不同时间，用ＤＮＡ片段化和细胞ＤＮＡ原位末端标记技术检测细胞凋亡情况。结果：紫外线照射Ｋ５６２ｎｅｏ和Ｋ５６２ｐ５３细胞８分钟后再培养７～１２小时，Ｋ５６２ｎｅｏ和Ｋ５６２ｐ５３两组细胞的凋亡差异显著。结论：野生型Ｐ５３蛋白在Ｋ５６２细胞表达后，能加速紫外线诱导的Ｋ５６２细胞凋亡。
Objective:To answer whether wild type p53 can sensitize K562 cell apoptosis induced by UV irradiation. Methods: K562 cells transducted with retrovirus encoding wild type p53 were irradiated by ultraviolet for different time and then cultured for different time. Apoptosis was detected by TDT end labelling technique, DNA fragmentation and MTT assay. Results: TDT end labelling and DNA fragmentation showed that apoptosis induced by 8 min UV irradiation differed significantly between K562 neo and K562 p53 cells. Conclusion: Wild type p53 can enhance apoptosis induced by UV irradiation on K562 cells
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1998年第12期631-633,共3页
Chinese Journal of Hematology
基金
国家自然科学基金
