摘要
以马铃薯栽培品种粤引85-38试管苗为材料进行微型薯诱导试验。结果表明:培养基(MS+8.0%蔗糖)中添加500 mg.L-1的矮壮素或5 mg.L-1的6-BA均能促进微型薯的形成,增加结薯数量和鲜薯产量,矮壮素和6-BA配合使用虽能提高微型薯的大薯率和鲜薯产量,但平均结薯数量却下降。温度在20~25℃有利于微型薯的形成和膨大;液体培养基诱导微型薯的效果优于固体培养基,是诱导微型薯较好的培养方式;经过壮苗的试管苗诱导微型薯效果优于未经壮苗的植株。
The tube shoots of the potato cultivar Yueyin 85-38 were used as the materials in the study. The results showed that the medium (MS+Sugar 8. 0% ) adding with Chlormequat Chloride(CCC)500 mg·L^-1 or 6-BA 5 mg·L^-1 could induce the formation of the micrcro-tuber and increase ks amount and the yield of fresh tubers. And the medium with CCC 500 mg·L^-1+6-BA 5 mg· L^-1 could increase the rate of big tubers and the weight of fresh tubers,but the number of growing tubers was decreased. The temperature between 20℃ and 25℃ was advantageous in forming and inflating the micro-tuber. The inductive efficiency of the liquid medium was higher than that of the solid medium,liquid medium was the best one to produce the tube miniature potatoes. The strong seedling of plantlet was suitable for inducting the micro-tuber.
出处
《黑龙江农业科学》
2009年第5期10-13,共4页
Heilongjiang Agricultural Sciences
基金
广东省科技计划项目(2006B20301003)
广东省财政厅项目(粤财农(2008)324号)
惠州市科技计划项目(2005P47)