摘要
目的探讨植物生长调节剂(NAA、2,4-D、6-BA、KT、PP333)对黄独微型块茎诱导形成的影响,以期找到适宜的微型块茎诱导形成的植物生长调节剂浓度和组合。方法采用植物组织培养技术、单因素试验和正交试验的方法,以黄独试管苗的带芽茎段为外植体,开展植物生长调节剂对黄独微型块茎诱导形成的影响等方面的研究。结果生长素单独使用有利于黄独微型块茎的诱导形成,NAA和2,4-D诱导微型块茎形成的适宜浓度均为0.5 mg/L,两者的诱导效果无显著性差异。细胞分裂素单独使用不利于黄独微型块茎的形成,KT和6-BA诱导微型块茎形成的适宜浓度均为2 mg/L,但KT的诱导效果好于6-BA。生长素、细胞分裂素和PP333组合可以显著促进黄独微型块茎的诱导形成,其中较佳的组合为MS+NAA 0.5 mg/L+6-BA 2.0 mg/L+PP333 0.5 mg/L。结论筛选出了诱导黄独微型块茎形成的植物生长调节剂单独使用和组合使用的适宜浓度,为黄独微型块茎的离体诱导形成及工业化生产奠定了技术基础。
Objective In order to find the suitable concentration and combination of plant growth regulators,the effects of plant growth regulators（NAA,2,4-D,6-BA,KT,and PP333） on in vitro induction formation for the plantlet microtuber of Dioscorea bulbifera was studied.Methods Through plant tissue culture technique,single factor test,and orthogonal test,taking the stems with a bud of D.bulbifera plantlets as explants,the effects of plant growth regulators on the in vitro induction formation for the microtubers of D.bulbifera were investigated.Results Auxin using alone was conducive to the induction formation for the microtuber of D.bulbifera.The suitable concentration of both NAA and 2,4-D inducing the microtuber formation was 0.5 mg/L,but the inducing effects of NAA and 2,4-D had no significant difference.Cytokinin using alone was not conducive to the induction formation for the microtuber of D.bulbifera.The suitable concentration of both KT and 6-BA inducing microtuber formation was 2 mg/L,but the inducing effect of KT is better than that of 6-BA.The combination of auxin,cytokinin,and PP333 could significantly promote the in vitro induction formation for the microtuber of D.bulbifera,the better combination was MS＋NAA 0.5 mg/L＋6-BA 2.0 mg/L＋PP333 0.5 mg/L.Conclusion Based on these experimental results,the paper selects the suitable concentration of plant growth regulators conducive to the in vitro induction formation for the microtuber of D.bulbifera,which has laid the technical foundation for their in vitro induction formation of microtuber and factory production.
出处
《中草药》
CAS
CSCD
北大核心
2014年第13期1928-1937,共10页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(31360072)
江西省教育厅2014年度科学技术研究一般项目(GJJ14713)
关键词
植物生长调节剂
黄独
试管苗
微型块茎
离体诱导
plant growth regulators
Dioscorea bulbifera L.
plantlet
microtuber
in vitro induction
