摘要
以浙江省‘台州紫山药’试管苗腋芽为材料,研究了组培容器培养基用量、植物生长调节剂浓度配比、蔗糖浓度及光照强度对紫山药试管薯形成和生长发育影响。结果表明:1/2MS+6-BA 0.1mg·L-1+NAA 2.0 mg·L-1+0.02%活性炭+蔗糖70 g·L-1、光照强度2 000 lx及每个组培容器(ZP17-440)培养基用量为60 mL的培养条件,有利于紫山药试管薯的形成和生长发育,试管薯在培养40 d左右开始形成,90 d诱导率达100%。
With the axillary buds of in vitro plantlets of Dioscorea alata L. from Taizhou City, Zhejiang Province, the effect of microtuber formation and development was studied based on different medium volumes, plant growth regulator concentration ratio, sucrose concentration and illumination intensity. The results showed that the preferred condition for induction of microtuber was 1/2MS basal medium supplemented with 0.1 mg · L16-BA, 2.0 mg· L-1NAA, 70 g · L-1 sucrose and 0.02% activated carbon under 2 000 lx illumination intensity. The volume of 1/2MS was 60 mL for every tissue culture vessel which standard was ZP17-440. The microtuber began forming at the 40 d and the inductivity reached 100% in 90 d.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第10期1999-2005,共7页
Acta Horticulturae Sinica
基金
江苏省农业科技自主创新资金项目[CX(13)5008]
关键词
紫山药
组织培养
试管薯诱导
Dioscorea alata L. tissue culture microtuber induction
