摘要
目的:从黑灵芝中提取分离出粗多糖,纯化得到一种新型纯多糖,并对其纯度、理化性质及抗氧化活性进行了研究。方法:采用Sevag法脱除粗多糖中的游离蛋白,并分别采用乙醇分级沉淀以及Superdex-200凝胶柱层析法纯化多糖,经高效凝胶渗透色谱法检验纯度。分别对纯多糖的溶解性、热特性及光谱特性等多种理化性质进行测定。最后分别采用苯甲酸钠法、FRAP(还原能力)法以及β-胡萝卜素漂白法对黑灵芝多糖的羟基自由基清除能力,总抗氧化能力以及对β-胡萝卜素-亚油酸氧化体系的抑制能力进行了分析。结果:分离出一种纯度较高的多糖,且具有显著的抗氧化能力。结论:黑灵芝多糖具有显著的抗氧化活性。
In order to investigate physico-chemical characteristics and antioxidant activity of polysaccharide from Ganoderma atrum fruitbody, a pure polysaccharide was isolated and purified sequentially using Sevag method, ethanol grade precipitation and Superdex-200 gel filtration chromatography. The purity of this polysaccharide was identified by HPLC. Solubility, thermal stability and spectral properties of this polysaccharide were also evaluated. Meanwhile, scavenging capability of hydroxyl free radicals, total antioxidant capability and suppression ability in β-carotene linoleic acid oxidative system were analyzed. It was concluded that polysaccharide from Ganoderma atrum has distinct antioxidant activity, which provides a theoretical basis for the exploitation of a new natural anfioxidant.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第19期55-60,共6页
Food Science
基金
国家"863"计划项目(2008AA10Z325)
食品科学与技术国家重点实验室目标导向资助项目(SKLF-MB-200806)
江西省科技厅科技攻关重点项目
关键词
黑灵芝
多糖
理化性质
抗氧化活性
Ganoderma atrum
polysaccharide
physico-chemical characteristics
antioxidant activity
