摘要
以川西若尔盖草原所采的鲜大蓟为研究材料,通过正交试验进行超临界CO_2萃取,大蓟挥发油提取率为3.0%.采用GC-MS方法分析了该挥发油的化学组成,共鉴定出其中41个化学成分并测定其相对含量,占总含量的99.65%.其中,邻苯二甲酸双-2-乙基己酯(30.81%),邻苯二甲酸二异辛酯(16.60%),邻苯二甲酸单产-2-乙基己基酯(15.96%)为主要成分.体外抑菌实验表明,该挥发油对8种供试细菌和7种真菌均有抑制作用.
The essential oil of Cirsiurnjaponicurn DC. was extracted by supercritical CO2 fluid extraction （CO2-SFE）. About 41 kinds of constituents were identified in the volatile oil by GC-MS analysis. The major chemical constituents were 1,2-Benzenedicarboxylic acid, his （2-ethylhexyl） ester, accounting for 30. 81%, 1,2-benzenedicarboxylic acid diisoctyl ester and phthalic acid mono-2-ethylhexyl ester. The filter paper mensuration is adopted and also the tube culture to estimate its antibiotic activity among 8 kinds of tested germs in the antibiotic experiments. The results showed that the volatile oil got a broadspectrum antibiotic activity.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第5期1531-1536,共6页
Journal of Sichuan University(Natural Science Edition)
基金
国家科技支撑计划(2007BAC18B03)
