摘要
采用叶盘法将兔防御素NP－1基因导入毛白杨后，经PCR分析、Southern杂交检测与筛选获得了转基因植株。并经体外抑菌实验表明，转NP－1基因毛白杨植株组织提取物对某些微生物的生长具有明显的抑制作用。
Rabbit defensin NP-1 possesses a broad resistant spectrum to pathogens. In this work, we have transferred the NP-1 gene into poplar plants by Agrobacterium-mediated transformation. PCR amplification and Southern analysis showed that rabbit defensin NP-1 gene was integrated into the poplar genome. The transformahon efficiency is about 15.6%. Antimicrobial achvity test showed that the extract of transgenic plants inhibited the growth of the tested microbes.
基金
国家自然科学基金!39770627
