摘要
为了鉴定随机扩增产物的同源性以及更有效地利用DNA分子标记研究人参种质资源，我们首次将毛细管PCR扩增的RAPD反应产物进行限制性内切酶消化并有效的进行了酶切位点分析，结果证明PCR-RFLP是筛选人参特异DNA分子标记的一种简单易行的新方法，可为药用植物种质资源研究提供另一有用的工具。
篒n order to identify homogeneity of RAPD(random amplified polymorphic DNA) products and to make full use of RAPD markers for the study of ginseng germplasm,it was tried for the first time,to digest PCR products produced by capillary PCR and their restriction sites determined effectively. Results showed that PCR-RFLP was a simple and useful method for screening distinctive molecular markers on Panax ginseng C. A. Mey,which provided a new tool for the study of germplasm of medicinal plants.
出处
《中草药》
CAS
CSCD
北大核心
1998年第9期625-627,共3页
Chinese Traditional and Herbal Drugs
基金
中国科学院植物研究所系统与进化植物学开放实验室资助
