摘要
利用正交试验对中药黄芩中提取黄芩素酶解工艺进行了初步探讨,确定最佳酶解工艺条件:温度为40℃、pH6.0、时间12h时,黄芩素含量最高;利用酶活力法对黄芩素体外抑制弹性蛋白酶活性进行了研究,发现黄芩素对弹性蛋白酶具有明显的抑制作用,抑制率达92.98%。
The optimal enzymolysis procedure of baicalein from Scutellaria baicalensis Georgi was obtained as the temperature of enzymolysis for 40℃,pH 6.0,time is 12h. The content of baicalein is the highest under these conditions.The inhibition of Porcine Pancreatic elastase in vitro by baicalein was studied by enzyme activity.The baicalein is very strong in the capacity of the inhibition of Porcine Pancreatic elastase with the inhibition rate of 92.98%.
出处
《光谱实验室》
CAS
CSCD
北大核心
2009年第4期993-996,共4页
Chinese Journal of Spectroscopy Laboratory
基金
科技部科技支撑计划资助(2006BA106A20-10)
关键词
黄芩素
酶解
弹性蛋白酶
抑制作用
Baicalein
Enzymolysis
Porcine Pancreatic Elastase
Inhibition
