摘要
为揭示银杏花粉促进雌花生长发育的作用机理，试验研究了花粉和花粉植物激素粗提液对银杏雌花生长及其内部过氧化物酶同工酶变化的影响。结果表示：银杏雌花在“吐水”后５ｄ生长量达到高峰；在“吐水”时用花粉植物激素粗提液涂抹胚珠可使雌花在处理后５ｄ继续生长，但在第１０ｄ亦达到峰值，花粉携带的植物激素不足以促进雌花的生长与坐种。应用聚丙烯酰胺凝胶电泳分析表明，雌花在授粉前后过氧化物酶同工酶谱带没有增减；
In order to clarify the mechanism that pollinating promoted the growth of female flower, changes of the growth and isoperoxidase of female flower in ginkgo biloba treated with pollination and phytohormone extracts of pollen was observed. The results obtained were as follows: It was found that the weight of female flower in Ginkgo biloba reached its peak value on the 5th day after “guttation”,then it declined; female flower would continue to grow but reached its peak weight on the 10th day after “guttation”if it was treated with phytohormone extracts of pollen. The results indicated that phytohormones from pollen through pollinating to ovule couldn′t provide female flower enough quantity for its developing and seed set.With SDS PAGE, isozyme bands of peroxidase in female flower did′t change, however, compared with non pollinatied, the activity of peroxidase was lower in ovule,but higher in pedicel on the days after pollinating. It could thus be inferred that pollinating would maintain ovule at high lever of IAA and promote pedicel xylem differentiation.
出处
《江苏农学院学报》
CSCD
1998年第3期53-56,共4页
Jiangsu Agricultural Research
关键词
银杏
雌花
传粉
过氧化物酶同工酶
ginkgo
female flower
pollination
peroxidase isoenzyme
