摘要
目的:探讨肝核受体LXRs激动剂T0901317对正常人骨骼肌细胞中FAT/CD36基因mRNA表达的影响。方法:将原代培养的5例成人骨骼肌细胞分为用肝核受体LXRs激动剂T0901317(1μmol/L)作用组、T0901317(0.5μmol/L)作用组和阴性对照组,作用24h后采用SYBR Green I实时荧光定量PCR法检测各组成人骨骼肌细胞FAT/CD36基因mRNA表达水平,并用2-△△Ct方法进行比较分析。结果:①以浓度为1μmol/L的T0901317作用组和0.5μmol/L的T090131作用组和对照组样本的均数进行方差分析,差别有统计学意义(P<0.01)。②浓度为1μmol/L的T0901317作用组和0.5μmol/L的T090131作用组成人骨骼肌细胞中FAT/CD36基因的mRNA表达分别是对照组的3.03倍和2.91倍。结论:肝核受体LXRs激动剂T0901317能够提高成人骨骼肌细胞中FAT/CD36基因mRNA的表达水平,提示T0901317有加快骨骼肌细胞内脂肪酸的堆积作用,推测LXRs激动剂T0901317可能会增加糖尿病患者骨骼肌胰岛素抵抗的风险。
Aim： To investigate the liver X receptors agonists T0901317＇ s effect on expression of FAT/CD36 gene mRNA in adult human skeletal muscle cell. Methods： Myotubes from humans were exposed to different T0901317 concentrations（0, 0.5, and 1.0μmol/L） for 24 hours before experiments were performed. Then the expression of FAT/CD36 mRNA in skeletal muscle cell of each experimental group was detected by SYBR Green Ⅰ real-time quantitative polymerase chain reaction. The relative data were compared among groups by 2^-△△Ct method. Results： ①The Ct mean of control group,T0901317（0.5 μmol/L） group, T0901317（1 μmol/L） group were analyzed and there was significant differenee（P 〈 0.01）.②The expression of FAT/CD36 mRNA with liver X receptors agonists T0901317 in human skeletal muscle cell in the 39901317（0.5/.anol/L） group and T0901317（1/nnol/L） group were 2.91 times and 3.03 times than the control group. Conclusion： The expression of FAT/CD36 mRNA in human skeletal muscle cell afer the treatment of fiver X receptors agonists T0901317 is increased, so we may propose that T0901317 may increase the risk of resistance in adult human skeletal muscle.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2009年第3期428-432,共5页
Chinese Journal of Applied Physiology
关键词
肝核受体
骨骼肌细胞
脂肪酸转位酶基因
定量PCR
LXRs
skeletal muscle cell
FAT/CD36 gene
real-time quantitative polymerase chain reaction
