摘要
目的:观察升清降浊方对皮下注射谷氨酸钠(MSG)大鼠骨骼肌的影响,以探索其改善胰岛素抵抗的作用机制。方法:新生大鼠皮下注射MSG并高脂诱导,制造胰岛素抵抗肥胖大鼠,选择48只MSG肥胖模型大鼠,雌雄各半。将大鼠随机分为6组:模型组,菲诺贝特100 mg.kg-1剂量组,罗格列酮5 mg.kg-1剂量组,升清降浊方6,3,1.5 g.kg-1剂量组;选正常鼠8只,雌雄各半做为正常对照组。给药50 d后,观察受试药物对MSG肥胖模型大鼠骨骼肌中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)及糖元含量的影响。结果:升清降浊方对MSG肥胖模型大鼠骨骼肌糖脂代谢有明显改善作用,与模型组相比,给药组大鼠骨骼肌中TC,TG,LDL-C及糖元含量明显降低,升清降浊方3个剂量组具有极显著性差异(P<0.01);给药组骨骼肌中HDL-C含量明显升高,升清降浊方3 g.kg-1剂量组具有极显著性差异(P<0.01)。结论:升清降浊方能明显改善MSG大鼠骨骼肌组织中脂质和糖原含量。
Objective：To observe the effects of Shengqing Jiangzhuo Fang（SQJZF） on skeletal muscle in monosodium L-glutamate（MSG） obese rats with insulin resistance.Method： The rat MSG-obese model was produced by giving neonatal rats subcutaneous injection of monosodium glutamate and induced with high-fat diet.Sixty MSG rats were divided into seven grous（4 male and 4 female per gtoup） randomly.the rosiglitazone group,the fenofibrate group,high dose,middle dose and low dose of SQJZF.After the administration for 50 days,levels of cholesterol（TC）,triglyceride（TG）,low density lipoprotein cholesterin（LDL-C）,high density lipoprotein cholesterin（HDL-C） and glucogn in muscle tissue were measured.Result：SQJZF showed good effects on the animal models.Compared with the model group,treatment with SQJZF at a dose of 6,3,1.5 g · kg-1 for 50 d decreased levels of TC,TG,LDL-C and glucogn in muscle tissue（P＆lt;0.01）;levels of HDL-C was significantly elvated by treatment with SQJZF at a dose of 3 g · kg-1（P＆lt;0.01）.Conclusion：SQJZF may contribute to ameliorate the glucolipid metablization in diabetic skeletal muscle.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第9期201-203,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81173373)
