摘要
目的:建立仙灵骨葆胶囊(淫羊藿、丹参、补骨脂、地黄等)中淫羊藿苷和淫羊藿定C的含量测定方法。方法:采用RP-HPLC法测定含量。色谱柱为Spherisorb C18(4.6mm×250mm,5μm),以乙腈-水为流动相梯度洗脱,流速为1.0mL/min,检测波长为270nm,柱温为25℃。结果:淫羊藿定C在0.22～2.20μg内其含量与峰面积呈良好的线性关系,平均回收率为103.2%,RSD为3.1%。淫羊藿苷在0.04～0.40μg内其含量与峰面积呈良好的线性关系,平均回收率为97.8%,RSD为3.2%。结论:本方法分析时间短且重现性和稳定性较好,可作为仙灵骨葆胶囊的质量控制方法。
AIM：To set up a method for determining epimedin C and icariin in Xianling Guobao Capsules（Herba Epimedii,Radix et Rhizoma Salviae miltiorrhizae,Fructus Psoraleae,Radix Rehmanniae,etc.）. METHODS：The chromatographic conditions included the column of Spherisorb C 18 （4.6 mm×250 mm,5 μm）,the mobile phase was acetonitrice and water as gradient eluent was at a flow rate of 1.0 mL/min,the detection wavelength was set at 270 nm and the column temperature was at 25 ℃. RESULTS：The linear range of epimedin C was 0.22-2.20 μg and icariin was 0.04-0.40 μg, respectively. The average recovery of epimedin C and icariin were 103.2% （RSD =3.1% ） and 97.8% （RSD =3.2% ）, respectively. CONCLUSION： The method is reliable, stable and well reproducible, and can control the quality of Xianling Guobao Capsules.
出处
《中成药》
CAS
CSCD
北大核心
2009年第8期1211-1213,共3页
Chinese Traditional Patent Medicine
基金
贵州省中药现代化项目(黔科合农字[2005]5034号)资助
国家863计划项目(SQ200802LS1479332)资助
