摘要
目的建立补肾益气颗粒中补骨脂素和异补骨脂素的含量测定方法。方法Diamonsil C18柱（200mm×4．6mm，5μm），流动相为甲醇-水（55：45），流速0．85ml／min，检测波长245nm。结果补骨脂素的线性范围为0．08～0．8μg，r=0．99998（n=6），平均加样回收率为99．69％，RSD为2．27％；异补骨脂素的线性范围为0．0796—0．796μg，r=0．99998（n=6），平均加样回收率为100．25％，RSD为2．82％。结论该法简便，准确，重现性好，可使补骨脂素和异补骨脂素达到基线分离。可以控制补肾益气颗粒的质量。
Objective To establish a method for the determination of psoralen and isopsoralen in Bushenyiqi granules. Methods The Diamonsil C18 （200 mm ×4.6 mm ,5μm）was used. The mobile phase consisted of methanol -water（ 55：45 ）. The flow rate was 0.85 ml · min^-1. The UV detective wavelength was 245 nm. Results The linear range of psoralen was 0.08 - 0.8 μg, r = 0.999 98 （ n = 6 ） with an average recovery rate of 99.69% （ RSD = 2.27% ） , the liner range of isopsoralen was 0.079 6 - 0. 796μg,r =0. 999 98 （n = 6） with an average recovery rate of 100.25% （RSD = 2.82% ）. Conclusion The method can be used to control the quality of Bushenyiqi granules with such advantages as simple,reliable and good reproducibility.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第2期373-374,共2页
Lishizhen Medicine and Materia Medica Research
