摘要
为了解优质鸡MEF2A基因SNPs与屠体性状的相关性,以240只大恒优质肉鸡为材料,应用12对引物,采用PCR-SSCP技术对肌细胞特异性增强子因子2A(MEF2A)基因进行多态性检测。结果在该研究群体中检测到MEF2A基因的3个SNPs位点46 023 nt(C→T,SNP1)、72 626 nt(G→A,SNP2)和89 232 nt(G→T,SNP3)。关联分析结果表明,MEF2A基因3个SNPs位点及其双倍型对部分屠体性状有显著(P<0.05)或极显著(P<0.01)影响。最小二乘分析结果表明,SNP1中,基因型A2A2个体的活体质量、屠体质量、胸肌质量和腹脂质量最小二乘均值显著(P<0.05)或极显著(P<0.01)大于A1A1个体;SNP2中,基因型B1B1个体的活体质量、屠体质量、胸肌质量、腿肌质量和腹脂质量的最小二乘均值显著(P<0.05)或极显著(P<0.01)大于B2B2个体;SNP3中的基因型C1C1个体的半净膛率显著高于基因型C2C2个体(P<0.05)。双倍型H1H2组合的活体质量、屠体质量、胸肌质量和腿肌质量的最小二乘均值均高于其他双倍型组合。H5H5双倍型的活体质量、屠体质量和胸肌质量的最小二乘均值均为最低。初步推断MEF2A基因可以作为影响鸡屠体性状的分子标记。
To understand the relationship between SNPs of MEF2A gene and carcass traits in chicken, three SNPs loci in MEF2A gene were detected by PCR-SSCP method in 240 Daheng quality meat chicken and its genetic effects on 9 carcass traits were also analyzed. Three polymorphic sites were C/T mutation at nt 46 023, G/A mutation at nt 72 626 and G/T mutation at nt 89 232, respectively. Statistical analysis showed that the three SNPs and diplotypes significantly associated with partial carcass traits （P〈0.05 or P〈0.01）. The multiple-range test indicated that A2A2 chicken of SNP1 had higher live weight, carcass weight, breast muscle weight, breast muscle percentage （P〈0. 01）, abdominal fat weight （P〈0.05） than that of A1A1 chicken; B1B1 chicken of SNP2 had higher live weight, carcass weight, breast muscle weight （P〈0.01）, leg muscle weight and abdominal fat weight （P〈0.05） than that of B2B2 chicken; CIC1 chicken of SNP3 had significantly higher semi-eviscerated percent than that of C2C2 chicken. The LSM of live weight, carcass weight, breast muscle weight and leg muscle weight of diplotype H1H2 were higher than that of all the other diplotypes, while the HSH5 diplotype had the lowest LSM of live weight, carcass weight and breast muscle weight in all the diplotypes. It was concluded that MEF2A gene can be regarded as a useful candidate gene for carcass traits of quantity meat chicken.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第8期1164-1170,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
十一五国家科技支撑项目(2008BADB2B01)
四川省科技厅生物技术项目资助(05NG002-017)
