摘要
目的观察针刺对急性脑缺血再灌注大鼠脑内IL-6 mRNA基因表达规律的影响。方法应用大鼠脑缺血再灌注模型,采用原位杂交方法观察脑缺血再灌注后假手术组、对照组与针刺组再灌注2h、6h、12h、24h各时间点IL-6 mRNA基因表达的变化,同时应用透射电镜观察对照组及针刺组再灌注24h后大鼠缺血区额顶叶皮质超微结构。结果假手术组大鼠IL-6 mRNA在皮层、纹状体呈基础水平表达,对照组缺血区皮层、纹状体于缺血后2hIL-6 mRNA表达开始升高,于24h达高峰。且纹状体区表达较皮层强烈。阳性细胞多位于梗死区周围。除在缺血再灌注后2h外,针刺2组在缺血区皮层、纹状体区IL-6 mRNA各时间点表达明显增强,与对照2组相比具有显著性差异(P<0.05～0.01)。针刺对缺血再灌注后细胞核的变性,细胞器的坏死有明显的改善作用,可使线粒体,内质网结构恢复正常,恢复细胞核的完整性,与对照组有明显差异。结论针刺可明显增强皮层及纹状体IL-6 mRNA的表达。
Objective To observe the expression of IL-6 mRNA and study the mechanism of acupuncture in focal cerebral ischernia. Methods In situ hybridization was used to detect the expression of IL-6 rnRNA in the sham operation group, control group and ac upuncture group 2 h, 6 h, 12 h, 24 h after reperfusion in the brain of middle cerebral artery occlusion rats, meanwhile and also observe ultrastructure in cortex of ischemic brain tissue 24 h after reperfusion. Results The sham operation group showed a basic expression of IL-6 mRNA in cortex and striate body. The cells expressing IL-6 mRNA were increased 2 h after isehernic reperfusion in cortex and striate body and peaked 24 h after reperfusion in control group and acupuncture group. The cells expressing IL-6 rnRNA in cortex of ischemic area were stronger in acupuncture group than in control group at every research time point besides 2 h after ischemic reperfusion. The ceils expressing IL-6 mRNA striate body of isehemic area were stronger in acupuncture group than in control group at every research time point. Acupuncture can lighten the degeneration of cell nucleolus and necrosis of cell, resume the integrity of the nucleolus, improve the function of mitochondrial and endoplasrnic, and have significant difference with control group. Conclusion Acupuncture can increase the cells expressing of IL 6 mRNA.
出处
《中国康复理论与实践》
CSCD
2009年第8期736-739,共4页
Chinese Journal of Rehabilitation Theory and Practice
